C-22211
Endothelial Cell Growth Medium 2
Basal Medium, 500 ml
Synonym(s):
Primary Cell Culture Media, Recommended with C-39216 or C-39211
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.81
Recommended Products
form
aqueous solution
packaging
pkg of 500 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Endothelial Cell Basal Medium 2 (supplements sold separately)
To form a complete medium, it should be combined with the media supplements: C-39216 or C-39211
To form a complete medium, it should be combined with the media supplements: C-39216 or C-39211
Application
Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
Components
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Quality
All lots of PromoCell Endothelial Cell Media 2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Storage and Stability
Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C
Subculture Routine
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Certificates of Analysis (COA)
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