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A9231

Sigma-Aldrich

Acridine Orange solution

2% in H2O

Synonym(s):

3,6-Acridinediamine, N,N,N′,N′-tetramethyl-, monohydrochloride, 3,6-Bis(dimethylamino)acridine hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C17H19N3 · HCl
CAS Number:
65-61-2
Molecular Weight:
301.81
MDL number:
MFCD00005029
UNSPSC Code:
12171500
PubChem Substance ID:
329771043
NACRES:
NA.47

form

liquid

Quality Level

100

shelf life

18 mo.

concentration

2% in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

InChI

1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

InChI key

DPKHZNPWBDQZCN-UHFFFAOYSA-N

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General description

Acridine Orange (C.l. 46005) is an acridine dye with a ring structure and overall small planar conjugated system.

Application

Acridine orange is a DNA intercalating dye that is a recommended DNA stain for flow cytometry. It differentially stains double-stranded and single-stranded nucleic acids.

It also has the following applications-
  • staining of RNA and acid mucins
  • detecting apoptosis
  • staining blood smears to diagnose malaria
  • identifying and enumerating granulocytes in fish
  • staining eukaryotic cells to demonstrate low pH structures

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Matthew P Harris et al.
PLoS genetics, 4(10), e1000206-e1000206 (2008-10-04)
The genetic basis of the development and variation of adult form of vertebrates is not well understood. To address this problem, we performed a mutant screen to identify genes essential for the formation of adult skeletal structures of the zebrafish.
Marko Gerić et al.
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, 47(11), 1521-1527 (2012-06-19)
The present study aimed to characterize chemical properties of clay bricks containing 20 % of sewage sludge. After detection of potentially hazardous metals, we simulated precipitation exposure of such material to determine the amount of heavy metals that could leach
Q L Chen et al.
Talanta, 97, 48-54 (2012-07-31)
We have utilized various valving scheme to leverage purely rotation-regulated flow control to enable comprehensive cell-based bioassays (CBBs) on centrifuge-based lab-on-a-disc (LOAD). A LOAD has been developed to examine allergic degranulation from live basophils for allergens screening for the first
Donald P Evenson
Methods in molecular biology (Clifton, N.J.), 927, 147-164 (2012-09-21)
The SCSA(®) is the pioneering assay for the detection of damaged sperm DNA and altered proteins in sperm nuclei via flow cytometry of acridine orange (AO) stained sperm. The SCSA(®) is considered to be the most precise and repeatable test
Hong-xiang Wang et al.
Zhonghua yi xue za zhi, 92(26), 1828-1831 (2012-09-05)
To ascertain the epididymal sperm nuclear maturity in obstructive azoospermia (OA) patients. A total of 81 infertile males from andriatry clinic of Renji hospital were selected, including 32 OA patients (OA group) and 49 asthenospermia patients (asthenospermia group). Another 32
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