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A9019

Sigma-Aldrich

Activated CH-Sepharose™ 4B

lyophilized powder

Synonym(s):

5-Carboxypentyl-Sepharose™ 4B N-hydroxysuccinimide ester, 6-Aminocaproic acid N-hydroxysuccinimide ester-activated-Sepharose™ 4B, 6-Aminohexanoic acid N-hydroxysuccinimide ester-activated-Sepharose™ 4B, CH-activated Sepharose™ 4B

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About This Item

UNSPSC Code:
13111000

form

lyophilized powder

extent of labeling

10-14 μmol per mL

matrix

Sepharose 4B

matrix activation

cyanogen bromide

matrix attachment

amino group

matrix spacer

8 atoms (when ligands are coupled to the free carboxy groups. No coupling agent is necessary.)

swelling

1 g swells to 2.4-4.3 mL

storage temp.

2-8°C

Application

Activated CH-Sepharose 4B is a medium which binds to ligands containing amino groups via an 8 atom arm. It has been used with gel permeation chromatography to determine fractions of β-Glucans in different species of plant extract, which may display anti-diabetic activities.

Physical form

Lyophilized powder stabilized with lactose and dextran

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A C C F F De Paula et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 38(6), 885-893 (2005-06-04)
Beta-glucans are soluble fibers with physiological functions, such as interference with absorption of sugars and reduction of serum lipid levels. The objective of the present study was to analyze the distribution of beta-glucans in different tissues of the African grass
R P Gupta et al.
Brain research, 878(1-2), 32-47 (2000-09-21)
Diisopropyl phosphorofluoridate (DFP) is an organophosphorus ester, which produces organophosphorus ester-induced delayed neuropathy (OPIDN) in hen and other sensitive species. A single dose of DFP (1.7 mg/kg, sc.) produces mild ataxia in 7-14 days in hens, which develops into severe
G D McIntyre et al.
The Biochemical journal, 211(2), 519-522 (1983-05-01)
A new affinity column for renin was prepared by coupling the isosteric peptide inhibitor of renin, H.77 (D-His-Pro-Phe-His-LeuR-Leu-Val-Tyr, where R is a reduced isosteric bond, -CH2-NH-), to activated 6-aminohexanoic acid-Sepharose 4B. Chromatography of a crude extract of human kidney cortex
Yea-Woon Kim et al.
Biotechnology letters, 27(7), 483-487 (2005-06-02)
Beta-glucans were prepared from Agaricus blazei Murill by repeated extraction with hot water. The average molecular weights of beta-glucans were 30-50 kDa by gel filtration chromatography. Oligosaccharides (AO), derived from hydrolyzing beta-glucans with an endo-beta-(1-->6)-glucanase from Bacillus megaterium, were mainly
S J Cartwright et al.
The Biochemical journal, 221(2), 505-512 (1984-07-15)
Several beta-lactamases, enzymes that play an important part in antibiotic resistance, have been purified by affinity chromatography on boronic acid gels. The procedure is rapid, appears to be selective for beta-lactamases, and allows a one-step purification of large amounts of
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