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Sigma-Aldrich

Chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Synonym(s):

Chloroform:Isoamyl Alcohol Solvent Mix

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About This Item

MDL number:
MFCD00171265
UNSPSC Code:
12352401
PubChem Substance ID:
329752812
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioUltra

Assay

≥99.5% (chloroform + isoamyl alcohol, GC)

form

liquid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.001% free alkali (as NH3)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤0.5 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.1
λ: 280 nm Amax: 0.07

SMILES string

ClC(Cl)Cl.CC(C)CCO

InChI

1S/C5H12O.CHCl3/c1-5(2)3-4-6;2-1(3)4/h5-6H,3-4H2,1-2H3;1H

InChI key

BKHZIBWEHPHYAI-UHFFFAOYSA-N

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Application

Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions.

Components

mixture of chloroform and isoamyl alcohol in the ratio of 49:1 (v/v)

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Central nervous system, Liver,Kidney

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM2628
BCCK6256
BCCG9218
BCCF6185
BCCC4649

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Purification of RNA by SDS solubilization and phenol extraction.
Rio DC, Ares M Jr, Hannon GJ, Nilsen TW.
Cold Spring Harbor Protocols, 2010, 5439-5439 (2010)
D.M. Wallace
Methods in Enzymology, 152, 35-35 (1987)
Bacterial RNA isolation.
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Cold Spring Harbor Protocols, 1012, 1024-1027 (2012)
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