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18248

Sigma-Aldrich

Protein Disulfide Isomerase from bovine liver

≥150 U/mg protein (protein ca. 40%)

Synonym(s):

PDI

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About This Item

CAS Number:
37318-49-3
Enzyme Commission number:
5.3.4.1 (BRENDA, IUBMB)
MDL number:
MFCD00163364
UNSPSC Code:
12352204

form

powder
powder with small lumps

specific activity

≥150 U/mg protein (protein ca. 40%)

mol wt

107 kDa by gel filtration
 57 kDa by SDS-PAGE

shipped in

wet ice

storage temp.

−20°C

InChI

1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12)

InChI key

DHUYKLYJBKXDBM-UHFFFAOYSA-N

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Physical properties

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

Unit Definition

1 unit corresponds to the amount of enzyme, which, in a glutathione-dithiothreitol-system, causes a change of absorbance of 0.001 per minute at pH 4.0 and 25°C.

Other Notes

Enzyme that accelerates the exchange reaction between disulfide bridges in proteins

replaced by

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