ST1517

Anti-PIP5K3 Mouse mAb (6C7)

liquid, clone 6C7, Calbiochem^®

• Synonym(s): Anti-Phosphatidylinosi-3-Phosphate-Phosphatidylinosi-5-Kinase Type 3
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6C7, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PIKFYVE(200576)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~60 kDa PIP5K3 protein.

Recognizes the ~60 kDa PIP5K3 protein in human testes tissue and HeLa cells.

This Anti-PIP5K3 Mouse mAb (6C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of PIP5K3.

Immunogen

A recombinant polypeptide corresponding to amino acids 342-452 of human PIP5K3, expressed as a GST fusion protein

Human

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
Human testes tissue, HeLa cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Tsuruta, F., et al. 2009. J. Cell Biol.187 279.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg