ES-006
EmbryoMax® Gelatin Solution
0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
Synonym(s):
Porcine Gelatin Coating Solution, porcine gelatin
About This Item
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product name
EmbryoMax® 0.1% Gelatin Solution, The EmbryoMax 0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
Quality Level
100
200
form
liquid
manufacturer/tradename
Specialty Media
EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
Application
1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:
Wells | _ | Amount/Well |
---|---|---|
96 | 100 μL | |
48 | 300 μL | |
24 | 0.5 mL | |
12 | 1.0 mL | |
6 | 1.5-2.0 mL |
2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.
3. Remove the gelatin solution and discard.
4. Immediately add cells to the dish.
Note: Do not allow the dishes to dry before adding cells.
Physical form
Storage and Stability
Legal Information
Disclaimer
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
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Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
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