71545
BacMagic DNA Kit - Novagen
Synonym(s):
BacMagic-1 DNA Kit
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.51
Recommended Products
manufacturer/tradename
Novagen®
Quality Level
storage condition
OK to freeze
shipped in
wet ice
storage temp.
2-8°C
General description
The BacMagic-1 DNA Kit provides a positive selection for creating baculovirus recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac, pBAC, or pTriEx vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 DNA Kit contains the BacMagic-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.
Components
- 0.5 µg- BacMagic DNA
- 50 µl- Insect GeneJuice Transfection Reagent
- 2 µg- Transfection Control Plasmid
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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