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Millipore

BugBuster® (primary amine-free) Extraction Reagent

Synonym(s):

Protein Extraction Reagent

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About This Item

UNSPSC Code:
41106511
NACRES:
NA.77

form

liquid

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

ambient

storage temp.

10-30°C

Related Categories

General description

BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking. The PIPPS buffer used in BugBuster (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Features and Benefits

Primary Amine-Free Formulation: BugBuster® (primary amine-free) Extraction Reagent is specially designed for applications where primary amines would interfere with protein extract. This means that academic researchers can purify metal-dependent proteins or proteins to be used for immobilization or crosslinking without worrying about the presence of primary amines, which could cause interference.

Enhanced Extraction: The extraction process can be further enhanced by the addition of rLysozyme Solution and Benzonase Nuclease, which helps to efficiently extract and purify proteins from the sample.

Metal Ion Compatibility: The PIPPS buffer used in the primary amine-free formulation of BugBuster® has a similar buffer capacity and pH range as the original Tris-buffered BugBuster® but will not complex metal ions. This makes it an ideal choice for extraction of metal-dependent proteins

Warning

Toxicity: Standard Handling (A)

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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