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Sigma-Aldrich

Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor™ 488 conjugate

clone 1A6, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

1A6, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG2bκ

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)

Application

This Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor 488 conjugate is validated for use in FC, WB for the detection of Nitrotyrosine.

Quality

Routinely evaluated by immunoblot.

Physical form

Protein G Purified mouse monoclonal in a solution of 1% BSA, PBS, 0.05% Tween, and 0.05% sodium azide as a preservative.

Storage and Stability

Stable for 1 year at 2–8°C from date of shipment in undiluted aliquots. Upon receipt, and prior to removing the cap, centrifuge the vial to remove any material in the cap. DO NOT FREEZE. Store at 2–8°C in aliquots, in the dark. Avoid repeated warming/cooling cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biological selectivity and functional aspects of protein tyrosine nitration.
Ischiropoulos, Harry
Biochemical and biophysical research communications, 305, 776-783 (2003)
Compartmentalized nitrosation and nitration in mitochondria.
Ghafourifar, Pedram and Colton, Carol A
Antioxidants & Redox Signaling, 5, 349-354 (2003)
Relevance of protein nitration in brain injury: a key pathophysiological mechanism in neurodegenerative, autoimmune, or inflammatory CNS diseases and stroke.
Sarchielli, P, et al.
Amino Acids, 25, 427-436 (2003)
Kyung-Sun Heo et al.
The Journal of cell biology, 193(5), 867-884 (2011-06-01)
Atherosclerosis is readily observed in regions of blood vessels where disturbed blood flow (d-flow) is known to occur. A positive correlation between protein kinase C ζ (PKCζ) activation and d-flow has been reported, but the exact role of d-flow-mediated PKCζ
J Torreilles et al.
BMC immunology, 2, 1-1 (2001-03-07)
Peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combination of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine. The present article describes
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