1.15924

Nigrosine (C.I. 50420)

water-soluble for microscopy Certistain^®

• Synonym(s): Nigrosine (C.I. 50420)
• CAS Number: 101357-32-8
• UNSPSC Code: 41116130
• EC Index Number: 309-930-4

Properties

• Quality Level: 400
• form: solid
• pH: 10 (20 °C, 10 g/L in H₂O)
• solubility: 10 g/L
• bulk density: 750 kg/m³
• εmax: 200-300 at 570-580 nm in 50% ethanol at 0.025 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C

Description

General description

The dry dye Nigrosin (C.I. 50420) water-soluble for microscopy Certistain^®, is used for the preparation of a staining solution for the visualization of capsule (negative capsule staining) in bacteriological material of human origin. As a Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 570 - 580 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.025 g/l; ethanol 50 %): 200 - 300
TLC-Test: passes test
Loss on drying (110 °C): ≤ 15 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(C): Not applicable