907278
H-L-Photo-leucine HCl
≥98%
Synonym(s):
(S)-2-Amino-3-(3-methyl-3H-diazirin-3-yl)propanoic acid hydrochloride, (S)-2-Amino-3-(3H-diazirin-3-yl)butanoic acid hydrochloride, Diazirine amino acid, Photo-Leu, Photo-crosslinking amino acid, Photoprobe building block
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About This Item
Empirical Formula (Hill Notation):
C5H10ClN3O2
Molecular Weight:
179.60
UNSPSC Code:
12352209
Recommended Products
Assay
≥98%
form
solid
reaction suitability
reaction type: solution phase peptide synthesis
availability
available only in USA
application(s)
peptide synthesis
storage temp.
−20°C
Application
H-L-Photo-leucine HCl is a diazirine-containing leucine amino acid and multifunctional photo-crosslinker. Its incorporation into peptides or small-molecule probes and tools allows for photoaffinity labeling of cellular targets and protein-protein interactions upon UV light (∼360 nm) irradiation to form a covalent bond. This and other multifunctional probe building blocks will continue to accelerate drug discovery research for probing cellular mechanisms, target ID/validation, and understanding traditionally undruggable targets. An Fmoc-protected version is also available as 907391.
Other Notes
Development, optimization, and structural characterization of an efficient peptide-based photoaffinity cross-linking reaction
for generation of homogeneous conjugates from wild-type antibodies
Mechanistic studies of a small-molecule modulator of SMN2 splicing
Protein-Polymer Conjugation via Ligand Affinity and Photoactivation of Glutathione S-Transferase
Direct Interaction between an Allosteric Agonist Pepducin and the Chemokine Receptor CXCR4
Photo-leucine and photo-methionine allow identification of protein-?protein interactions in living cells
Fishing for Drug Targets: A Focus on Diazirine Photoaffinity Probe Synthesis
Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)
for generation of homogeneous conjugates from wild-type antibodies
Mechanistic studies of a small-molecule modulator of SMN2 splicing
Protein-Polymer Conjugation via Ligand Affinity and Photoactivation of Glutathione S-Transferase
Direct Interaction between an Allosteric Agonist Pepducin and the Chemokine Receptor CXCR4
Photo-leucine and photo-methionine allow identification of protein-?protein interactions in living cells
Fishing for Drug Targets: A Focus on Diazirine Photoaffinity Probe Synthesis
Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)
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Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Self-react. C
Storage Class Code
5.2 - Organic peroxides and self-reacting hazardous materials
WGK
WGK 3
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We report a protein labeling method using nonselective carbene reactions of sufficiently high efficiency to permit detection by mass spectrometric methods. The approach uses a diazirine-modified amino acid (l-2-amino-4,4'-azipentanoic acid, "photoleucine") as a label source, which is converted to a
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