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RAB0166

Sigma-Aldrich

Phospho-EGFR (pTyr1068) and pan-EGFR ELISA Kit

for detection of human phospho-egfr (pTyr1068) and pan-egfr in cell and tissue lysates

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type: human cell lysate
sample type: human tissue lysate

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EGFR(1965)

General description

The Phospho-EGFR (pTyr1068) ELISA (Enzyme-Linked Immunosorbent Assay) kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates.

Immunogen

EGFR (pTyr1068) synthetic phosphopeptide, Recombinant Human EGFR

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

EGFR (epidermal growth factor receptor) functions as a receptor for EGF-family of proteins, which are peptide growth factors. This receptor-ligand system is also involved in the pathogenesis and progression of various types of carcinomas. In cancer, EGFR pathways promotes cellular proliferation, angiogenesis, metastasis and apoptosis. The microRNA miR-218-5p interacts with and controls EGFR, and in non-small cell lung cancer (NSCLC), the levels of EGFR are inversely related to miR-218-5p. miR-218-5p inhibits proliferation and migration of lung cancer cells by binding with EGFR, and thus, has potential in future NSCLC therapies. Phospho-EGFR activates phospho-Akt pathway in invasive bladder cancer (BC) and it can be considered as a promising therapeutic target for cancer.

Other Notes

Phosphorylated and pan protien can both be measured , in one experimnet.

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Phospho-Akt pathway activation and inhibition depends on N-cadherin or phospho-EGFR expression in invasive human bladder cancer cell lines.
Wallerand H
Urologic Oncology, 28(2), 180-188 (2010)
Tumor-suppressive miR-218-5p inhibits cancer cell proliferation and migration via EGFR in non-small cell lung cancer.
Zhu K, et al.
Oncotarget, 7(19), 28075-28085 (2016)
A novel approach in the treatment of cancer: targeting the epidermal growth factor receptor.
Ciardiello F, Tortora G
Clinical Cancer Research, 7(10), 2958-2970 (2001)
Epidermal growth factor receptor (EGFR) signaling in cancer.
Normanno N
Gene, 366(1), 2-16 (2006)

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