L8918

Anti-LC3 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Lc3, LC3 Antibody - Anti-LC3 antibody produced in rabbit, Lc3 Antibody, Anti-MAP1 light chain 3-like protein 1, Anti-MAP1A/1B light chain 3A, Anti-MAP1A/1BLC3, Anti-MAP1A/MAP1B LC3A, Anti-MAP1ALC3, Anti-MAP1BLC3, Anti-MAP1LC3A, Anti-MAP1LC3B, Anti-Microtubule-associated protein 1 light chain 3 alpha, Anti-Microtubule-associated protein 1 light chain 3 beta, Anti-Microtubule-associated proteins 1A/1B light chain 3
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 16-18 kDa
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells; western blot: 1-2 μg/mL using whole extracts of rat and mouse brain
• UniProt accession no.: Q9H492
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAP1LC3A(84557) , MAP1LC3B(81631) ; mouse ... Map1lc3a(66734) , Map1lc3b(67443) ; rat ... Map1lc3a(362245) , Map1lc3b(64862)

Description

General description

The gene LC3, also referred to as MAP1LC3A (microtubule associated protein 1 light chain 3 α), is a homologue of Apg8p necessary for autophagy in yeast. The cytosolic form upon processing gets localized to the autophagosome membranes. The gene is mapped to human chromosome 20q11.22. LC3 exists in cells as cytoplasmic LC3-I (18kDa) and LC3-II (16kDa).

Immunogen

Synthetic peptide corresponding to amino acids of human LC3A isoform a, conjugated to KLH via C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-LC3 antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence
• immunohistochemistry
• filter retardation assay
• immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (3 papers)
Immunohistochemistry (1 paper)
Western Blotting (21 paper)

Biochem/physiol Actions

The gene MAP1LC3A (microtubule associated protein 1 light chain 3 α) encodes a protein that serves as a marker of autophagosomes. It is found to be expressed on surviving oligodendrocytes in Nasu-Hakola disease brains and may be involved in the induction of oligodendrogliopathy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves