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C7901

Sigma-Aldrich

Chelex® 100 sodium form

50-100 mesh (dry)

Synonym(s):

Iminodiacetic Acid Resin

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About This Item

CAS Number:
11139-85-8
MDL number:
MFCD00163980
UNSPSC Code:
23151817
NACRES:
NA.56

form

beads

Quality Level

200

matrix

1% cross-linked polystyrene
 cross-linked polystyrene beads

matrix active group

iminodiacetic acid (sodium form)
paired iminodiacetate groups

matrix attachment

through methyl group to aromatic rings

particle size

50-100 mesh (dry)

pH

4-14

capacity

≥0.3 meq/mL binding capacity
 0.6 meq/g binding capacity (heavy metal ions)

Application

Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents.
Chelex® 100 sodium form has been used:
  • for the extraction of DNA from bee samples
  • to remove from cigarette smoke extract (CSE) and study the effects of hepcidin expression in the murine lungs
  • to extract DNA from bacterial cultures

Biochem/physiol Actions

Chelex® 100 is a styrene-divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. It is considered a weakly acidic resin. Chelex acts as a chelating resin that is used in DNA extraction from forensic samples, such as hair, bloodstain cards, and buccal swabs.

Other Notes

Product is autoclavable in sodium form. It has a maximum operating temperature of 75°C and is not soluble in water.

Legal Information

Chelex is a registered trademark of Bio-Rad Laboratories, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Methods for extracting genomic DNA from whole blood samples: current perspectives
Chacon Cortes D, et al.
Journal of Biosciences, 2014(2), 1-9 (2014)
Elizabeth Perez et al.
Journal of immunology (Baltimore, Md. : 1950), 205(9), 2489-2498 (2020-09-23)
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Germini A, et al.
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T P Ryan et al.
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The effects of transition metals on nonenzymatic and ceruloplasmin catalyzed epinephrine oxidation were investigated by studying rates of epinephrine oxidation in purified buffers and in the presence of metal chelating agents. We found that epinephrine does not "autoxidize" in sodium
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