114359

Bromocresol Green

ACS reagent, Dye content 95 %

• Synonym(s): BCG; 2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]-3-methylphenol
• Empirical Formula (Hill Notation): C₂₁H₁₄Br₄O₅S
• CAS Number: 76-60-8
• Molecular Weight: 698.01
• Beilstein: 372527
• EC Number: 200-972-8
• MDL number: MFCD00005874
• UNSPSC Code: 12171500
• PubChem Substance ID: 24847166
• NACRES: NA.47

Properties

• grade: ACS reagent
• Quality Level: 200
• Assay: ≥95% (After Oxygen Combustion, silver nitrate titration)
• form: powder
• composition: Dye content, 95%
• visual transition interval: 3.8, yellow(passes test); 3.8-5.4, yellow to blue(passes test); 4.5, green; 5.4, blue
• mp: 225 °C (dec.) (lit.)
• λmax: 423 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1c(Br)c(O)c(Br)cc1C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4C
• InChI: 1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3
• InChI key: FRPHFZCDPYBUAU-UHFFFAOYSA-N

Description

General description

Bromocresol Green is a synthetic triphenylmethane dye that is anionic in nature. At pH 3.8-5.4, it changes color from yellow to blue-green, and is therefore, commonly used as a pH indicator.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.

• It is used widely as a vital stain to study blood-brain permeability.
• It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
• It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
• It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
• It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves