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Sigma-Aldrich

Rat/Human Brain Derived Neurotrophic Factor ELISA Kit

measures and quantifies BDNF levels in serum, plasma, cell culture supernatent or tissue homogenate

Synonym(s):

BDNF, Brain Derived Neurotrophic Factor

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

product name

ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA, This ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.

Quality Level

species reactivity

human, rat

manufacturer/tradename

ChemiKine
Chemicon®

technique(s)

ELISA: suitable

UniProt accession no.

application(s)

research use

detection method

colorimetric

shipped in

wet ice

Gene Information

human ... BDNF(627)

General description

ChemiKine Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat.

Test Principle:

With the ChemiKine BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin-enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration: i.e., the higher the OD the higher the BDNF concentration in the sample.

Application:

ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

For Research Use Only. Not for use in diagnostic procedures.

Application

Research Category
Neuroscience
This ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.
Used to detect/quantify: BDNF

Components

ChemiKine BDNF ELISA Plate: (Part No. 60238) Two Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.

Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.

Standard/Sample Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).

BDNF Standard (Recombinant Human): (Part No. 60237) Two vials (Lyophilized).

Biotinylated Mouse anti-Human BDNF Monoclonal Antibody: (Part No. 60583) One 25 μL vial.

Streptavidin-Enzyme conjugate: (Part No. 60582) One 50 mL vial of HRP conjugated Streptavidin.

TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3′,5,5′-tetramethylbenzidine in a proprietary buffer with enhancer.

Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution.

Storage and Stability

Precautions:

· Wash Buffer and Sample Buffer contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

· The instructions provided have been designed to optimize the kit′s performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


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Joshua T Reycraft et al.
Medicine and science in sports and exercise, 52(5), 1210-1217 (2019-12-10)
Brain-derived neurotrophic factor (BDNF) is an exercise-induced neurotropin mediating neuroprotection and synaptic plasticity. Although exercise intensity is implicated as a potentially important mediator of BNDF release after exercise, the optimal exercise stimulus (interval vs continuous) and intensity (submaximal vs supramaximal)
Val66met polymorphism and serum brain-derived neurotrophic factor levels in bipolar disorder.
J Tramontina et al.
Molecular psychiatry, 12(3), 230-231 (2007-02-28)
Nicola Mirabella et al.
Cell and tissue research, 323(3), 513-522 (2005-11-04)
Brain-derived neurotrophic factor (BDNF) is a growth factor belonging to the family of neurotrophins. Although neurotrophins in the male genital organs have been well documented, their role in the biology of these organs is far from clear. In particular, little
Viktorija Trajkovska et al.
Brain research bulletin, 73(1-3), 143-149 (2007-05-15)
Although numerous studies have dealt with changes in blood brain-derived neurotrophic factor (BDNF), methodological issues about BDNF measurements have only been incompletely resolved. We validated BDNF ELISA with respect to accuracy, reproducibility and the effect of storage and repeated freezing
Megan E Kozisek et al.
Neuropharmacology, 54(2), 251-257 (2007-12-01)
Major depressive disorder is a major health problem in adults and is now recognized as a substantial problem in children as well. Tricyclic antidepressants, including desipramine (DMI), are no better than placebo in treating childhood and adolescent depression, but are

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