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UHPLC/MS Analysis of Diazepam and Metabolites on Titan C18, Comparison of Flow Rate and Column Length

UHPLC/MS Analysis of Diazepam and Metabolites on Titan C18, Comparison of Flow Rate and Column Length application for UHPLC

材料

標準

产品编号
说明
价格

O-902

奥沙西泮标准液 溶液

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

N-905

去甲地西泮标准液 溶液

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

D-907

地西泮标准液 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-907

替马西泮标准液 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Titan C18, 2 cm x 2.1 mm I.D., 1.9 μm particles (577120-U)

mobile phase

[A] 0.1% formic acid; [B] acetonitrile; (60:40, A:B)

flow rate

various

temp.

35 °C

detector

TIC MRM (UV also runs in series)

injection

1 μL

sample

100 ng/mL in mobile phase

说明

分析報告

These two sets of chromatograms show separations on 100, 50, 30, and 20 mm x 2.1 mm I.D. columns and at different flow rates and demonstrate the utility of the 1.9 μm Titan particles. The highest grade solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.

法律資訊

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Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS