HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL^™ LC-18

column

SUPELCOSIL LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)

mobile phase

[A] methanol: [B] deionized water (98:2, A:B)

flow rate

2 mL/min

detector

UV, 325 nm (retinol, retinyl acetate) or 290 nm (tocopherols, tocopherol acetate)

injection

20 μL

sample

0.2-1 mg/mL each analyte in methanol