OSHA Method 42/47 and ASTM^® D5836: HPLC Analysis of Isocyanates on SUPELCOSIL^™ LC-8 after Collection/Desorption using ORBO^™-80

sample/matrix

calibration standard: isocyanate derivatives, 5 μg/mL in acetonitrile blank: 1-(2-pyridyl)piperazine (1-2PP) coated glass fiber filter desorbed in acetonitrile/DMSO, 9:1

adsorbent tube

ORBO-80 (20811)

column

SUPELCOSIL LC-8, 25 cm × 4.6 mm I.D., 5 μm particles (58297)

mobile phase

0.05 M ammonium acetate in water:acetonitrile, 70:30 (pH 6-6.2 with acetic acid)

flow rate

linear gradient, 0.7 mL/min to 2 mL/min in 15 min

detector

UV, 254 nm

injection

10 μL