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HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery® HS F5

HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery® HS F5 application for HPLC

材料

分析柱

产品编号
说明
价格

Discovery ® HS F5 HPLC 色谱柱

5 μm particle size, L × I.D. 5 cm × 4.6 mm

標準

产品编号
说明
价格

吗啡-3- β-D-葡糖醛酸 溶液

100 μg/mL in methanol: water ( 50:50(v/v)), ampule of 1 mL, certified reference material, Cerilliant®

吗啡标准液 溶液

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

吗啡-6- β-D-葡糖苷酸 溶液

100 μg/mL in methanol: water (1:1), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Discovery HS F5, 5 cm x 4.6 mm I.D., 5 μm particles (567513-U)

mobile phase

[A] 10 mM ammonium acetate, pH 6.8 (unadjusted); [B] acetonitrile

gradient

0% B for 1 min, to 30% B in 4 min, held 3 min

flow rate

1 mL/min

column temp.

40 °C

detector

UV, 280 nm

injection

10 μL

sample

50 μg/mL in final mobile phase

说明

法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany