HPLC Analysis of Diet Soda Additives on Ascentis^® Express C18

column

Ascentis Express C18, 3 cm x 4.6 mm I.D., 2.7 μm particles (53818-U)

mobile phase

20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min

pressure

3191 psi (220 bar)

flow rate

3 mL/min

column temp.

40 °C

detector

UV, 214 nm

injection

1 μL

sample

(Diet Soda 100 - 500 μg/mL in buffer)