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Merck
  • Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells.

Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells.

International journal of nanomedicine (2017-02-23)
Marketa Bacakova, Julia Pajorova, Denisa Stranska, Daniel Hadraba, Frantisek Lopot, Tomas Riedel, Eduard Brynda, Margit Zaloudkova, Lucie Bacakova
摘要

Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types.

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Sigma-Aldrich
Thrombin 来源于人类血浆, lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)
Sigma-Aldrich
(3aR,4S,5R,6aS)-(-)-六氢-5-羟基-4-(羟甲基)-2H-环戊并[b]呋喃-2-酮, 98%