The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

Journal of nanoscience and nanotechnology (2014-04-18)

Wei-Qiang Yu, Ling Xu, Fu-Qiang Zhang

摘要

The TiO2 nanotubes by anodization have been extensively studied for medical implant and orthopedic applications because of enhancing bone development. In the present study, a new nano-foveolae structure verified by SEM and AFM was prepared by simulating the nanotubes exfoliation from anodized Ti. MC3T3-E1 preosteoblasts were used to investigate the effects of new nanoscale surface on cell spreading, proliferation, and osteogenic gene expression. The new nano-foveolae structure supported preosteoblast better spreading, more filopodiaes shown by SEM (4 h), and lower proliferation (72 h) than the smooth Ti. By two weeks, the new nanoscale surface induced higher expression of osteogenic markers alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), and collagen I (COL I) with real-time RT-PCR compared to the control group. The results of this study suggest that the new nano-foveolae structure provides a favorable surface for functions of bone cells.

材料

货号

品牌

产品描述

366994

Sigma-Aldrich

钛, powder, <45 μm avg. part. size, 99.98% trace metals basis

348791

Sigma-Aldrich

钛, foil, thickness 0.127 mm, 99.7% trace metals basis

267503

Sigma-Aldrich

钛, foil, thickness 0.25 mm, 99.7% trace metals basis

268496

Sigma-Aldrich

钛, powder, −100 mesh, 99.7% trace metals basis

460400

Sigma-Aldrich

钛, wire, diam. 0.25 mm, 99.7% trace metals basis

268526

Sigma-Aldrich

钛, sponge, 3-19 mm, 99.5% trace metals basis

369489

Sigma-Aldrich

钛, foil, thickness 2.0 mm, 99.7% trace metals basis

348848

Sigma-Aldrich

钛, foil, thickness 0.025 mm, 99.98% trace metals basis

348805

Sigma-Aldrich

钛, foil, thickness 0.5 mm, 99.99% trace metals basis

767506

Sigma-Aldrich

钛, sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.995% trace metals basis

GF16031843

钛, mesh, 100x100mm, nominal aperture 0.19mm, wire diameter 0.23mm, 60x60 wires/inch, open area 20%, twill weave

GF29374005

钛, mesh, 100x100mm, nominal aperture 4.3mm, wire diameter 1.5mm, 4.4x4.4 wires/inch, open area 94%, platinized diamond mesh

266035

Sigma-Aldrich

钛, wire, diam. 1.0 mm, 99.99% trace metals basis

305812

Sigma-Aldrich

钛, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)

460397

Sigma-Aldrich

钛, foil, thickness 0.127 mm, ≥99.99% trace metals basis

267481

Sigma-Aldrich

钛, foil, thickness 0.25 mm, 99.99% trace metals basis

267902

Sigma-Aldrich

钛, wire, diam. 0.81 mm, 99.7% trace metals basis

348864

Sigma-Aldrich

钛, wire, diam. 0.5 mm, 99.99% trace metals basis

GF31127712

钛, rod, 10mm, diameter 50mm, 99.99+%

GF14990935

钛, tube, 1000mm, outside diameter 10.3mm, inside diameter 8.7mm, wall thickness 0.8mm, annealed, 99.6+%

GF46592949

钛, wire, straight, 1000mm, diameter 1.0mm, as drawn, 99.6+%

GF49264713

钛, tube, 200mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%

433667

Sigma-Aldrich

钛, evaporation slug, diam. × L 6.3 mm × 6.3 mm, ≥99.99% trace metals basis

GF86399106

钛, tube, 500mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%

GF62977540

钛, tube, 200mm, outside diameter 2.03mm, inside diameter 1.63mm, wall thickness 0.2mm, annealed, 99.6+%

GF13900205

钛, tube, 100mm, outside diameter 0.51mm, inside diameter 0.35mm, wall thickness 0.08mm, annealed, 99.6+%

GF90963928

钛, rod, 25mm, diameter 50mm, 99.99+%

GF95731960

钛, tube, 500mm, outside diameter 3.0mm, inside diameter 1.5mm, wall thickness 0.75mm, annealed, 99.6+%

GF96834493

钛, rod, 1000mm, diameter 5mm, annealed, 99.6+%

GF04311638

钛, rod, 100mm, diameter 6mm, annealed, 99.6+%