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Merck

V900412

Sigma-Aldrich

三(羟甲基)甲基甘氨酸

Vetec, reagent grade, ≥99%

别名:

N-三(羟甲基)甲基甘氨酸

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About This Item

线性分子式:
(HOCH2)3CNHCH2CO2H
CAS号:
分子量:
179.17
Beilstein:
1937804
EC號碼:
MDL號碼:
分類程式碼代碼:
12161700
PubChem物質ID:

等級

reagent grade

產品線

Vetec

化驗

≥99%

形狀

crystalline powder

有用的pH值範圍

7.4-8.8

pKa (25 °C)

8.1

SMILES 字串

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI 密鑰

SEQKRHFRPICQDD-UHFFFAOYSA-N

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一般說明

三(羟甲基)甲基甘氨酸是最常用的电泳缓冲液,其负电荷比甘氨酸低。这反过来又有助于它更快地迁移。三(羟甲基)甲基甘氨酸也用于细胞团块的再悬浮。此外,它的高离子强度导致更多的离子运动和更少的蛋白质运动。这有助于在较低百分比的丙烯酰胺凝胶中分离低分子量蛋白质。与甘氨酸-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)系统相比,三(羟甲基)甲基甘氨酸也被用作拖尾离子,有助于在较低丙烯酰胺浓度下解析小蛋白质。

應用

用于分离低分子量的肽的缓冲剂组分。

法律資訊

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Encyclopedia of Dairy Sciences (2011)
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The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
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Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in
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Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Christian Nilsson et al.
Electrophoresis, 31(3), 459-464 (2010-02-02)
Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in

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