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Merck

G005952

HPLC Analysis of Fusion Protein on Zenix®-C SEC-300, Effect of Mobile Phase Composition

application for HPLC

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About This Item

技術

HPLC: suitable

測試參數

column: Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
gradient: (A) lower, 150 mM sodium phosphate buffer, pH 7.0 (B) middle, 150 mM sodium phosphate buffer, pH 7.0 + 10% acetonitrile (C) top, 150 mM sodium phosphate buffer, pH 7.0 + 20 mM NaCl
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 214 nm
injection: 10 μL
sample: 1 mg/mL fusion protein, MW 170 kD, pl 6.8-7.0

適合性

application for HPLC

應用

life science and biopharma

法律資訊

Zenix is a registered trademark of Sepax Technologies

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