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Merck

G005447

HPLC Analysis of Withania (Aswagandha) Standard on Ascentis® Express F5

application for HPLC

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About This Item

技術

HPLC: suitable

測試參數

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 20 to 100% B in 10 min: held at 100% B for 0.5 min
flow rate: 0.3 mL/min
pressure: 2756 psi (190 bar)
column temp.: 35 °C
detector: UV, 227 nm
injection: 5 μL
sample: 20 μg/mL in 80:20, water:methanol

適合性

application for HPLC

應用

vitamins, nutraceuticals, and natural products

分析報告

This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of withania standards.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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