技術
HPLC: suitable
測試參數
column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 20 to 100% B in 10 min: held at 100% B for 0.5 min
flow rate: 0.3 mL/min
pressure: 2756 psi (190 bar)
column temp.: 35 °C
detector: UV, 227 nm
injection: 5 μL
sample: 20 μg/mL in 80:20, water:methanol
適合性
application for HPLC
應用
vitamins, nutraceuticals, and natural products
分析報告
This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of withania standards.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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