产品名称
Ascentis® Phenyl 高效液相色谱柱, 5 μm particle size, L × I.D. 25 cm × 10 mm
物料
stainless steel column
Agency
suitable for USP L11
产品线
Ascentis®
特点
endcapped
制造商/商品名称
Ascentis®
包装
1 ea of
标记范围
19% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
长度 × 内径
25 cm × 10 mm
表面积
450 m2/g
表面覆盖度
5.2 μmol/m2
杂质
<5 ppm metals
基质
fully porous particle
silica gel high purity, spherical
基质活性基团
phenyl phase
粒径
5 μm
孔径
100 Å
工作pH范围
2-8
应用
food and beverages
分离技术
reversed phase
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一般描述
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis Phenyl 苯基柱在反相模式（包括 100% 水性条件）下具有出色的分离能力。也可用于 HILIC/ANP（水性正相）模式，并在梯度应用时紫外/质谱流失低。
Ascentis Phenyl 苯基柱在反相模式（包括 100% 水性条件）下具有出色的分离能力。也可用于 HILIC/ANP（水性正相）模式，并在梯度应用时紫外/质谱流失低。
特点和优势
- 100% 水性兼容
- ANP/HILIC 和反相
- 梯度条件下的低紫外/质谱流失
- 交替选择性
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
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