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Merck

567530-U

Supelco

Discovery® HS F5 (3 µm) HPLC Columns

L × I.D. 5 cm × 4 mm, HPLC Column

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery ® HS F5 HPLC 色谱柱, 3 μm particle size, L × I.D. 5 cm × 4 mm

材料

stainless steel column

agency

suitable for USP L43

產品線

Discovery®

特點

endcapped

製造商/商標名

Discovery®

包裝

1 ea of

標籤範圍

12% Carbon loading

參數

≤70 °C temp. range
400 bar pressure (5801 psi)

技術

HPLC: suitable
LC/MS: suitable

長度 × 內徑

5 cm × 4 mm

表面積

300 m2/g

表面覆盖率

4 μmol/m2

雜質

<10 ppm metals

基質

silica gel, high purity, spherical particle platform
fully porous particle

基質活性組

PFP (pentafluorophenyl) phase

粒徑

3 μm

孔徑

120 Å

工作pH值範圍

2-8

應用

food and beverages

分離技術

reversed phase

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相关类别

一般說明

将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则:
通常,碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来,使得大部分的 C18 方法可轻松转换到 F5 上。

特點和優勢

  • 独特的选择性
  • 保留能力与 C18 柱类似(有时需要洗脱能力更强的流动相)
  • 峰形极佳
  • 稳定性好、低流失,适用于 LC-MS 分析
  • 粒径范围从 3μm 到 10μm 可供选择

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法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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