ZRB1932

Anti-TBKBP1 Antibody, clone 3F13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: TANK-binding kinase 1-binding protein 1;TBK1-binding protein 1
• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 3F13, recombinant monoclonal
• 描述: 3F13 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 67.7 kDa; observed mol wt ~75 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: mouse, porcine, canine, rat, bovine, feline
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_055541
• UniProt登錄號: A7MCY6
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TBKBP1(9755)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 3F13 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects TANK-binding kinase 1-binding protein 1 (TBKBP1). It targets an epitope within 17 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human TANK-binding kinase 1-binding protein 1 (TBKBP1).

應用

Quality Control Testing

Evaluated by Western Blotting in NTERA-2 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TBKBP1 in NTERA-2 cell lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound TBKBP1 peptide with a KD of 2.1 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TBKBP1 in human placenta tissue sections.

Western Blot Analysis: A 1:1000 dilution of a representative lot of the this antibody detected TBKBP1 in HEK293T cells transfected with a TBKBP1 expression vector.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

TANK-binding kinase 1-binding protein 1 (UniProt: A7MCY6; also known as TBK1-binding protein 1) is encoded by the TBKBP1 (also known as KIAA0775, SINTBAD) gene (Gene ID: 9755) in human. TBKBP1 is an adapter protein that constitutively binds TANK-binding kinase 1 (TBK1) and Inhibitor of NF-kB kinase subunit epsilon (IKBKE) and plays a role in antiviral innate immunity. Its TBK1 and IKBKE interacting region is localized in amino acids 280-329. It can exist as a homodimer or can form heterodimer with NAP1. Its homodimerization region is localized in amino acids 1-279. TBKBP1 expression is observed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen, muscle, heart, and brain. TBK1 that is reported to be a central mediator of growth factor signaling relies on TBKBP1 for its action. TBKBP1 recruits TBK1 to protein kinase C (PKC ) through a scaffold protein, caspase recruitment domain 10 (CARD10). This enables PKC to phosphorylate TBK1 at serine 716. This phosphorylation is essential for TBK1 activation by growth factors, but not by innate immune stimuli. It has been reported that although the TBK1-TBKBP1 signaling axis is not essential for the induction of type I interferon, it facilitates mTORC1 activation and oncogenesis. Deletion of either TBK1 or TBKBP1 is known to inhibit tumorigenesis in a murine model of lung cancer. The TBK1-TBKBP1 axis can also facilitate tumor-mediated immunosuppression via induction of PD-L1 and stimulation of glycolysis. Two isoforms of TBKBP1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhu, L., et al. (2019). Nat. Cell Biol. 21; 1604-1614; Goncalves, A., et al. (2011). PLoS One. 6(9); e23971).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable