推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3G12, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 66.32 kDa
observed mol wt ~56 kDa
純化經由
using Protein A
物種活性
human, rat
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
同型
IgG
表位序列
N-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 3G12 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects GLS2. It targets an epitope with in 22 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal region of human Glutaminase liver isoform, mitochondrial (GLS2).
應用
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 1:1,000 dilution of this antibody detected GLS2 in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: 1:1,000 dilution from a representative lot detected GLS2 in rat liver, human liver, and human brain tissue lysate.
Flow Cytometry Analysis: 1 μg from a representative lot detected GLS2 in one million HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GLS2 in HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound GLS2 with a KD of 4.3 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 1:1,000 dilution of this antibody detected GLS2 in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: 1:1,000 dilution from a representative lot detected GLS2 in rat liver, human liver, and human brain tissue lysate.
Flow Cytometry Analysis: 1 μg from a representative lot detected GLS2 in one million HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GLS2 in HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound GLS2 with a KD of 4.3 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-GLS2, clone 3G12 ZooMAb®, Cat. No. ZRB1473, is a recombinant Rabbit monoclonal antibody that specifically detects GLS2 (L-glutaminase) and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.
標靶描述
Glutaminase liver isoform, mitochondrial (UniProt: Q9UI32; also known as EC:3.5.1.2, GLS, L-glutaminase, LGA, L-glutamine amidohydrolase, GLS2) is encoded by the GLS2 (also known as GA) gene (Gene ID: 27165) in human. Glutaminase, a key metabolic enzyme that hydrolyzes glutamine to glutamate and ammonia, is expressed as either kidney type (GLS) or liver type (GLS2). GLS2 is a homotetrameric protein that exists as a long and a short isoform and the long isoform is localized to the mitochondria. A third variant, which is much shorter than the others has also been reported, but it lacks the glutaminase catalytic domain. Although GLS2 is predominant in the liver, its expression has also been reported in the brain and pancreas. It promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and a-ketoglutarate. It also increases cellular antioxidant function via NADH and glutathione production. Both kidney and liver glutaminases are activated by phosphate. However, GLS2 is activated by lower concentrations of phosphate than GLS, but phosphate is ultimately able to increase GLS catalytic activity by a greater magnitude than for GLS2. In addition, GLS2 is also activated by ammonia, whereas ammonia inhibits GLS. High expression of GLS2 has been reported in a variety of human cancers, including bladder, breast, cervix, colon, kidney, liver, and lung. Inhibition or genetic silencing of GLS2 is shown to diminish the growth of A549 and HepG2 hepatoma cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Dias, MM., et al. (2020). Oncogene 39; 690-702; Katt, WP., et al. (2017). Future Med. Chem. 9(2); 223-243).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 产品选型工具.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
商品
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门