ZRB1145

Anti-HSP60 Antibody, clone 1N3, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1N3, recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 61.01 kDa; observed mol wt ~61 kDa
• 物種活性: human, rat, mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable (peptide)
• 同型: IgG
• UniProt登錄號: P10809
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HSPD1(3329)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 1N3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Heat shock protein 60. It targets an epitope within 65 amino acids from the C-terminal half.

免疫原

GST/HIs-tagged recombinant fragment corresponding to 65 amino acids from the C-terminal half of human Heat Shock Protein 60, isoform 1.

應用

Anti-HSP60, clone 1N3 ZooMAb , Cat. No. ZRB1145, is a highly specific rabbit recombinant monoclonal antibody, that targets Heat Shock Protein 60 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HSP60 in C6 and NIH/3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HSP60 in human kidney and mouse kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HSP60in HUVEC and HeLa cells.

Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

標靶描述

60 kDa heat shock protein, mitochondrial (UniProt: P10809; also known as 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein) is encoded by the HSPD1 (also known as HSP60) gene (Gene ID: 3329) in human. Hsp60 is a homoheptameric, highly conserved molecular chaperone present in the mitochondrial matrix where along with Hsp10 it helps in protein folding. It also prevents misfolding of proteins and promotes refolding. It is transcribed by a nuclear gene, translated in the cytosol and the newly translated protein is imported into mitochondria. Its mitochondrial import signal (MIS) is localized in the 26 amino acids at the N-terminus. Following its import, the MIS peptide is cleaved off to generate the mature protein. Its mitochondrial import depends on the mitochondrial membrane potential. Under certain pathological conditions, such as cancer and chronic inflammatory diseases, Hsp60 can also accumulate in the cytosol via a mitochondrial export mechanism. Two isoforms of Hsp60 have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Vilasi, S., et al. (2014). PLoS One. 9(5); e97657).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable