出版資訊
J. Sambrook and D. Russell, Cold Spring Harbor Laboratory Press, 2006, 700 pp., soft cover
製造商／商標名
Cold Spring Harbor
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一般說明
Molecular Cloning: Condensed Protocols is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. The book contains only the step-by-step portions of the protocols, accompanied by the appendices. Each protocol is cross-referenced to the appropriate pages in the original book.
This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world′s best-selling manual of molecular biology techniques.
This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world′s best-selling manual of molecular biology techniques.
目錄
1. Using Plasmid Vectors in Molecular Cloning
2. Bacteriophage λ and its Vectors
3. Working with Bacteriophage M13 Vectors
4. Working with High Capacity Vectors
5. Gel Electrophoresis of DNA and Pulsed Field Agarose Gel Electrophoresis
6. Preparation and Analysis of Eukaryotic Genomic DNA
7. Extraction, Purification and Analysis of mRNA from Eukaryotic Cells
8. In Vitro Amplification of DNA by the Polymerase Chain Reaction
9. Preparation of Radiolabeled DNA and RNA Probes
10. Working with Synthetic Oligonucleotide Probes
11. Preparation of cDNA Libraries and Gene Identification
12. DNA Sequencing
13. Mutagenesis
14. Screening Expression Libraries
15. Expression of Cloned Genes in Escherichia coli
16. Introduction of Cloned Genes into Cultured Mammalian Cells
17. Analysis of Gene Expression in Mammalian Cells
18. Protein Interaction Technologies
Appendices
Index
2. Bacteriophage λ and its Vectors
3. Working with Bacteriophage M13 Vectors
4. Working with High Capacity Vectors
5. Gel Electrophoresis of DNA and Pulsed Field Agarose Gel Electrophoresis
6. Preparation and Analysis of Eukaryotic Genomic DNA
7. Extraction, Purification and Analysis of mRNA from Eukaryotic Cells
8. In Vitro Amplification of DNA by the Polymerase Chain Reaction
9. Preparation of Radiolabeled DNA and RNA Probes
10. Working with Synthetic Oligonucleotide Probes
11. Preparation of cDNA Libraries and Gene Identification
12. DNA Sequencing
13. Mutagenesis
14. Screening Expression Libraries
15. Expression of Cloned Genes in Escherichia coli
16. Introduction of Cloned Genes into Cultured Mammalian Cells
17. Analysis of Gene Expression in Mammalian Cells
18. Protein Interaction Technologies
Appendices
Index
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