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應用
由于在缺乏特定培养基成分(比如氨基酸、嘌呤或嘧啶)的情况下无法生长,营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化,可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基,可以提高产量和生长速度,并增加筛选文库或进行基因敲除时转化的成功率。
成分
除非另有说明,否则补充剂包含以下所有成分:
氨基酸:除亮氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76mg/L。
其他营养物质:腺嘌呤(18 mg/L),肌醇(76 mg/L),对氨基苯甲酸(8 mg/L),尿嘧啶(76 mg / L)。
氨基酸:除亮氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76mg/L。
其他营养物质:腺嘌呤(18 mg/L),肌醇(76 mg/L),对氨基苯甲酸(8 mg/L),尿嘧啶(76 mg / L)。
其他說明
氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。
數量
1 l 培养基将补充 1.6 g。
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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