V7259

Vascular Endothelial Growth Factor human

VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture

• 别名: VEGF
• CAS号: 127464-60-2
• MDL號碼: MFCD00286542
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 生物源: human
• 品質等級: 200
• 重組細胞: expressed in E. coli
• 化驗: ≥98% (SDS-PAGE)
• 形狀: powder
• 效力: 1-10 ng/mL ED₅₀/EC₅₀
• 品質: endotoxin tested
• 分子量: protein 38.2 kDa (as homodimer)
• 包裝: pkg of 10 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: ≤0.1 EU/μg
• 顏色: white
• 溶解度: water: soluble
• UniProt登錄號: P15692
• 儲存溫度: −20°C
• 基因資訊: human ... VEGFA(7422)

说明

一般說明

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.

應用

Vascular Endothelial Growth Factor (VEGF) has been used for-

• the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
• inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

生化／生理作用

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

物理性質

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

外觀

Lyophilized from a sterile filtered buffered solution with no additives.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)