重組細胞
expressed in E. coli
品質等級
形狀
solution
分子量
~32 kDa
濃度
~5 units/μL
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... GTF2B(2959)
生化/生理作用
General transcription factor for RNA Polymerase II.
The amino terminal region of TFIIB contains a zinc ribbon fold composed of antiparallel Β strands. Mutational studies show that this domain is required for recruitment of RNA Pol II for transcriptional initiation.
單位定義
One gel shift unit is the amount of TFIIB required to form 0.2 pmol of TFIIB/TFIID/DNA complex as detected by gel shift.
外觀
Solution in 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, 0.1% Triton® X-100.
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves
Genes & development, 14(6), 719-730 (2000-03-25)
The function of the conserved zinc-binding domains in the related Pol II- and Pol III-specific factors TFIIB and Brf was investigated. Three-dimensional structure modeling and mutagenesis studies indicated that for both factors, the functional surface of the zinc ribbon fold
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