SRP5082

QIK, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 别名: DKFZp434K1115, KIAA0781, LOH11CR1I, SIK2, SNF1LK2
• UNSPSC代码: 12352200
• NACRES: NA.32

属性

• 重组: expressed in baculovirus infected Sf9 cells
• 产品线: PRECISIO^® Kinase
• 方案: ≥70% (SDS-PAGE)
• 表单: buffered aqueous glycerol solution
• 比活: 95-129 nmol/min·mg
• 分子量: ~150 kDa
• NCBI登记号: NM_015191
• 运输: dry ice
• 储存温度: −70°C
• 基因信息: human ... SIK2(23235)

说明

一般描述

QIK is a serine/threonine protein kinase that contains an N-terminal kinase domain, a central domain with ubiquitin-associate motif, and a C-terminal PKA phosphorylation site. QIK can phosphorylate IRS1 and overexpression of QIK in adipocyte elevates the phosphorylation of IRS1. The QIK-mediated phosphorylation of IRS1 may modulate the efficiency of insulin signal transduction and could be responsible for insulin resistance associated with diabetes. Insulin disrupts TORC2 activity by induction of QIK which then stimulates the phosphorylation and cytoplasmic translocation of TORC2. Phosphorylated TORC2 is subsequently degraded by the 26S proteasome.

外形

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

制备说明

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

法律信息

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable