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Merck

SRP3149

Sigma-Aldrich

sCD14 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

别名:

soluble CD14

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

生物源

human

重組細胞

expressed in HEK 293 cells

化驗

≥95% (HPLC)
≥95% (SDS-PAGE)

形狀

lyophilized

分子量

41.6 kDa

包裝

pkg of 50 μg

技術

cell culture | mammalian: suitable

雜質

<0.1 EU/μg endotoxin, tested

顏色

white

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... CD14(929)

序列

TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P

外觀

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Time to abandon dogma: CD14 is expressed by non-myeloid lineage cells.
Jersmann HP
Immunology and Cell Biology, 83(5), 462-467 (2005)
Proteolysis of monocyte CD14 by human leukocyte elastase inhibits lipopolysaccharide-mediated cell activation.
Le-Barillec K
The Journal of Clinical Investigation, 103(7), 1039-1046 (1999)
Richard L Kitchens et al.
Journal of endotoxin research, 11(4), 225-229 (2005-09-24)
LPS binding protein (LBP) and CD14 play key roles in promoting innate immunity to Gram-negative bacteria by transferring LPS to the signaling receptor complex, MD-2/Toll-like receptor 4 (TLR4). LBP and soluble CD14 (sCD14) can also inhibit responses to LPS by
Binding of CD14 to Mycoplasma genitalium-derived lipid-associated membrane proteins upregulates TNF-a.
He J
Inflammation, 37(2), 322-330 (2014)
CD14 protein acts as an adaptor molecule for the immune recognition of Salmonella curli fibers.
Rapsinski GJ
The Journal of Biological Chemistry, 288(20), 14178-14188 (2013)

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