生物来源
human
重组
expressed in insect cells
方案
≥80% (SDS-PAGE)
表单
frozen liquid
分子量
~56 kDa
包装
pkg of 5 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
500 μg/mL
颜色
clear colorless
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... TOP1(7150)
生化/生理作用
Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. The core domain spanning from amino acids 215 to 636 is highly conserved and retains DNA binding activity. Topo I has been found to nick the DNA with a preference of 5′-(A/T)(G/C)(A/T)T-3′. Camptothecin and its analogs have been tested as potent anticancer compounds by stabilizing topo I-DNA complex, thereby inhibiting both DNA and RNA synthesis.
外形
Clear and colorless frozen liquid solution
制备说明
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
L Stewart et al.
The Journal of biological chemistry, 271(13), 7593-7601 (1996-03-29)
Amino acid sequence comparisons of human topoisomerase I (Topo I) with seven other cellular Topo I enzymes reveal that the enzyme can be divided into four major domains: the unconserved NH2-terminal domain (24 kDa), the conserved core domain (54 kDa)
M R Redinbo et al.
Science (New York, N.Y.), 279(5356), 1504-1513 (1998-03-21)
Topoisomerases I promote the relaxation of DNA superhelical tension by introducing a transient single-stranded break in duplex DNA and are vital for the processes of replication, transcription, and recombination. The crystal structures at 2.1 and 2.5 angstrom resolution of reconstituted
J J Champoux
Annual review of biochemistry, 70, 369-413 (2001-06-08)
DNA topoisomerases solve the topological problems associated with DNA replication, transcription, recombination, and chromatin remodeling by introducing temporary single- or double-strand breaks in the DNA. In addition, these enzymes fine-tune the steady-state level of DNA supercoiling both to facilitate protein
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