SRP2015

RNA Polymerase II, p14.5 subunit human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• 别名: RPB9, hRPB14.5
• 分類程式碼代碼: 12352202
• NACRES: NA.26

属性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥80% (SDS-PAGE)
• 形狀: frozen liquid
• 分子量: ~16.6 kDa
• 包裝: pkg of 10 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: 300 μg/mL
• 技術: western blot: suitable
• 顏色: clear colorless
• NCBI登錄號: NM_006233
• UniProt登錄號: P36954
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... POLR2I(5438)

说明

生化／生理作用

hRPB9 is a subunit unique to RNA polymerase II, although it has sequence homologues in RNA polymerases I and III. The gene for Rpb9 is not essential for yeast cell viability, but is essential in Drosophila. hRPB9 has roles in both transcription initiation and transcription elongation. In the initiation reaction it is necessary for accurate start site selection. In the elongation reaction, RPB9, along with TFIIS facilitates the conversion of an arrest-competent conformation to a read-through competent conformation. RNA polymerase II lacking the RPB9 subunit uses alternate transcription initiation sites in vitro and in vivo and is unable to respond to the transcription elongation factor TFIIS in vitro. A role in the modulation of initiation and elongation is consistent with the localization of RPB9 in the three-dimensional structure of yeast RNA polymerase II. RPB9 is located at the tip of the so-called "jaws" of the enzyme, which is thought to function by clamping the DNA downstream of the active site. RPB9 comprises two zinc ribbon domains joined by a conserved linker region. The C-terminal zinc ribbon is similar in sequence to that found in TFIIS.

外觀

Clear and colorless frozen liquid solution

準備報告

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable