重組細胞
expressed in baculovirus infected Sf9 cells
化驗
70-95% (SDS-PAGE)
形狀
frozen liquid
比活性
5.0-7.0 nmol/min·mg
分子量
~66 kDa
濃度
0.05 μg/μL
登錄號
NM_178170
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... NEK8(284086)
應用
Useful for kinase assay and Western blot.
生化／生理作用
Recombinant human NEK8 (1-322) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK8 or NIMA-related kinase 8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans which may play a role in cell cycle progression from G2 to M phase. NEK8 is part of a complex of ciliary proteins required for renal and cardiovascular development (1). NEK8 interacts with polycystin signal transduction pathways and may control the targeting of these ciliary proteins (2).
重構
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
儲存和穩定性
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
法律資訊
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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