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Merck

SRP0198

Sigma-Aldrich

PARP11 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

别名:

C12orf6, DKFZp779H0122, MIB006, Poly (ADP-ribose) Polymerase 11

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

生物源

human

重組細胞

expressed in baculovirus infected insect cells

化驗

≥80% (SDS-PAGE)

形狀

aqueous solution

分子量

65.6 kDa

包裝

pkg of 10 μg

濃度

>0.02 mg/mL

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... PARP11(57097)

一般說明

Human PARP11 (GenBank Accession No. NM_020367), full length with N-terminal GST tag, MW = 65.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into acid-insoluble form.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


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Mirella L Meyer-Ficca et al.
Biology of reproduction, 92(3), 80-80 (2015-02-13)
Sperm are highly differentiated cells characterized by their species-specific nuclear shapes and extremely condensed chromatin. Abnormal head shapes represent a form of teratozoospermia that can impair fertilization capacity. This study shows that poly(ADP-ribose) polymerase-11 (ARTD11/PARP11), a member of the ADP-ribosyltransferase

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