SRE0019

三磷酸腺苷双磷酸酶 来源于马铃薯

Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein

• 别名: 腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶
• CAS号: 9000-95-7
• EC 号: 3.6.1.5 (BRENDA, IUBMB)
• EC號碼: 232-569-8
• MDL號碼: MFCD00130542
• 分類程式碼代碼: 12352204
• NACRES: NA.32

属性

• 品質等級: 400
• 種類: Grade VII
• 形狀: lyophilized powder
• ATPase activity: ≥200 units/mg protein
• secondary activity: ≥50 % of base activity ADPase
• 成份: Protein, 25-60%
• 溶解度: H₂O: soluble 1.0 mg/mL
• 應用: diagnostic assay manufacturing
• 異物活動: Acid Phosphatase ≤2% of base activity
• 儲存溫度: −20°C

说明

應用

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.

不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶：一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应：ATP → ADP+P_i → AMP+2P_i。

生化／生理作用

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

單位定義

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

外觀

Partially purified, lyophilized powder containing potassium succinate buffer salts.

準備報告

按 ATP 酶单位销售。

其他說明

该等级具有低 ATP 酶/ADP 酶比率。

安全信息

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H334
• 防範說明: P261 - P284 - P501
• 危險分類: Resp. Sens. 1
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable