SAB4200582

Anti-Epsin1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 别名: Anti-EPN1
• UNSPSC代码: 12352203
• NACRES: NA.44

属性

• 生物来源: rabbit
• 偶联物: unconjugated
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~90 kDa
• 种属反应性: rat, human, mouse
• 浓度: ~1.0 mg/mL
• 技术: immunoblotting: 1-2 μg/mL using extracts of SH-SY5Y cells and of mouse brain (S1 fraction).; immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat brain.; indirect immunofluorescence: 2-4 μg/mL using NRK cells.
• UniProt登记号: Q9Y6I3
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... EPN1(29924) ; mouse ... Epn1(13854) ; rat ... Epn1(117277)

说明

一般描述

There are three known mammalian epsin variants including epsin 1, 2 and 3. Epsin1 protein contains an epsin N-terminal homology (ENTH) region, a single clathrin-binding LVDLD motif and two or three tandem ubiquitin-interacting motifs (UIMs). The central region of epsin contains a clathrin interaction motif as well as repeats of a DPW motif, which binds to adaptor protein (AP)-2.

免疫原

synthetic peptide corresponding to an internal sequence of human epsin1 isoform A, conjugated to KLH. The corresponding sequence is identical in human espin1 isoforms B and C, and in rat and mouse epsin1.

应用

Anti-Epsin1 antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

生化/生理作用

Epsin1 (also known as EPN1) is an endocytic adaptor protein that plays a central role in the clathrin-mediated internalization and degradation of ubiquinated proteins. Epsin1 recruits ubiquitinated epidermal growth factor receptor (EGFR) into clathrin-coated pits. Epsin1 is required in mitotic membrane and spindle organization. The epsin family of endocytic adaptors is found to be upregulated in cancer and epsin1 overexpression promotes cancer cell invasion.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable