SAB4200413

Monoclonal Anti-LIN28 antibody produced in mouse

clone L28.5, tissue culture supernatant

• 别名: Monoclonal Anti-CSDD1, Monoclonal Anti-LIN28A, Monoclonal Anti-ZCCHC1
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: tissue culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: L28.5, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~28 kDa
• 物種活性: mouse, human
• 技術: western blot: 1:8,000-1:16,000 using extracts of Ntera-2 cells.
• 同型: IgM
• UniProt登錄號: Q9H9Z2
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... LIN28A(79727) ; mouse ... Lin28a(83557)

说明

一般說明

LIN28 belongs to a family of RNA-binding proteins that are mainly expressed in embryonic stem cells.

免疫原

synthetic peptide corresponding to a sequence at the C-terminus of human LIN28A. The isotype is determined using a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

應用

Monoclonal Anti-LIN28 antibody produced in mouse is suitable for immunoblotting at a working dilution of 1:8000 to 1:16000. For immunofluorescence, a dilution of 1:1500 to 1:3000 is recommended.

Monoclonal Anti-LIN28 antibody produced in mouse specifically recognizes C-terminus of human LIN28A.

生化／生理作用

The structure of LIN28 includes RNA-binding domains, an N-terminal cold-shock domain and a C-terminal Zn-knuckle domain. It inhibits the biogenesis of let-7 miRNA and modulates the translation of target mRNA. The expression of LIN28 is linked to the cellular processes of development, differentiation, oncogenesis and glucose metabolism. It is also linked to the resistance of breast cancer cells to radiation effects.

外觀

Solution containing 15 mM sodium azide.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable