生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~90 kDa
种属反应性
human, mouse
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
浓度
~1.5 mg/mL
技术
immunoprecipitation (IP): 10-20 μg using HEK-293T cell lysates overexpressing mouse BRSK1.
indirect immunofluorescence: 0.2-0.4 μg/mL using HEK-293T cells overexpressing mouse BRSK1.
western blot: 1-2 μg/mL using mouse forebrain (S1 fraction).
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... BRSK1(84446)
mouse ... Brsk1(381979)
一般描述
BRSK1 is a serine/threonine kinase that facilitates the G(2)/M cell cycle arrest upon UV- or methyl methane sulfonate-induced DNA damage. This kinase also modulates the polarity of neurons and duplication of centrosomes . Anti-BRSK1 (C-terminal) antibody is specific for mouse and human BRSK1. In immunoblotting, detection of the BRSK1 band is specifically inhibited by the BRSK1 immunizing peptide.
免疫原
synthetic peptide corresponding to a sequence at the C-terminal of human BRSK1, conjugated to KLH. The corresponding sequence is identical in mouse BRSK1.
应用
Anti-BRSK1 (C-terminal) antibody is suitable for use in western blot (1-2 μg/mL using S1 fraction extracts of mouse forebrain). The product can also be used for immunoprecipitation (10-20 μg) and indirect immunofluorescence (0.2-0.4 μg/mL) using HEK-293T cells overexpressing mouse BRSK1.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
María Alvarado-Kristensson et al.
Nature cell biology, 11(9), 1081-1092 (2009-08-04)
Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a
Rui Lu et al.
The Journal of biological chemistry, 279(30), 31164-31170 (2004-05-20)
Checkpoint activation by DNA damage during G(2) prevents activation of cyclin B/Cdc2 complexes, and as a consequence, mitotic entry is blocked. Although initiation and maintenance of G(2) arrest are known to be regulated by at least two distinct signaling pathways
Myriam Müller et al.
Journal of cell science, 123(Pt 2), 286-294 (2009-12-23)
Wee1 is well characterized as a cell-cycle checkpoint kinase that regulates the entry into mitosis in dividing cells. Here we identify a novel function of Wee1 in postmitotic neurons during the establishment of distinct axonal and dendritic compartments, which is
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