SAB4200380

Anti-LAYN antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 别名: Anti-LAYN antibody produced in rabbit, Anti-Layilin
• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: rabbit
• 偶联物: unconjugated
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~43 kDa
• 种属反应性: human
• 浓度: ~1.0 mg/mL
• 技术: indirect immunofluorescence: 2.5-5.0 μg/mL using human A549 cells.; western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human LAYN.
• UniProt登记号: Q6UX15
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... LAYN(143903)

说明

一般描述

Layilin (LAYN) is a cell surface hyaluronan receptor, originally identified as a binding partner of talin located in membrane ruffles of spreading cells. LAYN is widely expressed in different cell lines and tissues. This gene is mapped to human chromosome 11q.

免疫原

synthetic peptide corresponding to an internal region of human LAYN, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

应用

Anti-LAYN antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

生化/生理作用

Layilin (LAYN) binds to hyaluronan, a ubiquitous extracellular matrix component involved in the invasion of a variety of tumour cells, through its extracellular domain and to cytoskeleton-membrane linker proteins including Merlin and Radixin, through its cytoplasmic domain, implicating LAYN in cell adhesion and motility. LAYN is overexpressed in the human lung carcinoma A549. LAYN down-regulation by RNA interference, inhibits invasion and lymphatic metastasis of A549 cells.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

安全信息

• 储存分类代码: 10 - Combustible liquids
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable