生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~30 kDa
种属反应性
human
包装
antibody small pack of 25 μL
浓度
~1.0 mg/mL
技术
western blot: 1-2 μg/mL using Whole extracts of HEK-293T cells overexpressing human CPSF4.
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CPSF4(10898)
mouse ... CPSF4(54188)
rat ... CPSF4(304277)
一般描述
Cleavage polyadenylation specificity factor 4 (CPSF4), also called CPSF 30 is a component of the CPSF complex. It comprises of five CCCH zinc finger motifs (ZF1-ZF5). CPSF4 gene is mapped to human chromosome 7q22.1.
特异性
Anti-CPSF4 recognizes human CPSF4.
应用
Anti-CPSF4 antibody produced in rabbit has been used in chromatin immunoprecipitation and immunoblotting.
生化/生理作用
Cleavage polyadenylation specificity factor 4 (CPSF4) is a component of 3′-end mRNA processing machinery and binds to poly(U) sequence of RNA via the zinc finger motifs. These motifs are also involved in protein-protein interactions. CPSF4 along with other proteins primarily coordinate both cleavage and polyadenylation. Their interaction with the non-structural protein 1 (NS1) protein of the influenza virus leads to inhibition of polyadenylation event and 3′ end cleavage of pre-mRNA associated with the host. Elevated expression of CPSF4 is observed in lung adenocarcinomas.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Upregulation of cleavage and polyadenylation specific factor 4 in lung adenocarcinoma and its critical role for cancer cell survival and proliferation
Chen W, et al.
PLoS ONE, 8(12), e82728-e82728 (2013)
Jennelle C Hodge et al.
Genes, chromosomes & cancer, 48(10), 865-885 (2009-07-16)
Uterine leiomyomata (UL), the most common neoplasm in reproductive-age women, have recurrent cytogenetic abnormalities including interstitial deletion of 7q. To develop a molecular signature, matched del(7q) and non-del(7q) tumors identified by FISH or karyotyping from 11 women were profiled with
Lee Davidson et al.
Genes & development, 28(4), 342-356 (2014-01-31)
3' end formation of pre-mRNAs is coupled to their transcription via the C-terminal domain (CTD) of RNA polymerase II (Pol II). Nearly all protein-coding transcripts are matured by cleavage and polyadenylation (CPA), which is frequently misregulated in disease. Understanding how
3? end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells
Davidson L, et al.
Genes & Development, 28(4), 342-356 (2014)
C R Mandel et al.
Cellular and molecular life sciences : CMLS, 65(7-8), 1099-1122 (2007-12-26)
Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3'-end. Processing at the 3'-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplicity
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