S1577

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human

recombinant, expressed in E. coli, lyophilized powder, ≥97% (SDS-PAGE), suitable for cell culture

• 别名: SDF-1, SDF1, SDF-1α
• UNSPSC代码: 12352202
• NACRES: NA.32

属性

• 生物来源: human
• 质量水平: 200
• 重组: expressed in E. coli
• 方案: ≥97% (SDS-PAGE)
• 表单: lyophilized powder
• 效能: ≤50 ng/mL EC₅₀
• 包装: pkg of 10 μg
• 储存条件: avoid repeated freeze/thaw cycles
• 技术: cell culture | mammalian: suitable
• 杂质: ≤1 EU/μg protein Endotoxin
• 颜色: white
• UniProt登记号: P48061
• 运输: dry ice
• 储存温度: −20°C
• 基因信息: human ... CXCL12((6387)

说明

一般描述

Stromal Cell-Derived Factor 1α (SDF-1α) or pre-B Cell Growth Stimulation Factor (PBSF) is an 8 kDa chemokine peptide. SDF-1 was identified using signal sequence trap cloning. With this method, cDNAs have been cloned using mouse and human stromal cell lines. This cytokine is widely accepted as a CXC chemokine of the intercrine-macrophage inflammatory protein superfamily. The gene has been mapped to chromosome 10q and is encoded by 4 exons. Expression of the human SDF1 gene is abundant in the pancreas, spleen, ovary, and small intestine. The presence of a GC-rich sequence and the lack of a TATA box in the 5∅-flanking region of the gene are consistent with ubiquitous expression.

生化/生理作用

SDF-1α functions as a pre-B cell growth factor in the presence of IL-7 (interleukin-7) and a potent chemoattractant for T-lymphocytes and monocytes. It has also been found to be a ligand for CXCR4 (chemokine receptor type 4) or the orphan receptor LESTR/fusin (putative chemokine receptor). By signaling through the receptor, SDF1 may serve as an inhibitor of HIV-1 (human immunodeficiency virus), which utilizes the LESTR/fusin receptor as a point of entry. The SDF-1/CXCR4 signalling pathway is associated with cancer cell proliferation, migration and survival. Overexpression of SDF1 is observed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 offers CXCR4 mediated neuroprotection during post ischemic stroke.

外形

This product is lyophilized from a 0.2 μm-filtered solution containing 10 μg SDF-1α and 0.5 mg bovine serum albumin in PBS.

重悬

Reconstitute the contents of the vial using sterile buffer or medium containing a minimum of 0.1% BSA or HSA to a stock concentration of ≥10 μg/ml. Additional filtration of the stock solution is not recommended as this may result in loss of product due to adsorption onto the filter membrane.

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable