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生物源
mouse
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
4D11, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~68 kDa
物種活性
bovine, newt, chicken, human, monkey, mouse
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of MDBK cells
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... HNRNPL(3191)
mouse ... Hnrnpl(15388)
一般說明
Monoclonal Anti-hnRNP-L (mouse IgG1 isotype) is derived from the 4D11 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALA/c mice immunized with hnRNP proteins and purified by affinity chromatography on ssDNA-agarose. RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. Group L and M are one of the most abundant groups of proteins.
免疫原
hnRNP proteins.
應用
Anti-hnRNP-L antibody has been used in
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunocytochemistry
- western blotting
生化／生理作用
Heterogeneous nuclear ribonucleoproteins-L (hnRNP-L) is involved in splicing regulations, internal translation initiation of hepatitis C virus (HCV), stability of the human vascular endothelial growth factor (VEGF) mRNA under hypoxic growth conditions, and intron independent mRNA export.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.
Liu X and Mertz J E
Genes & Development, 9(14), 1766-1780 (1995)
Auto-and cross-regulation of the hnRNP L proteins by alternative splicing
RossbachO, et al.
Molecular and cellular biology, 29(6), 1442-1451 (2009)
Regulation of human vascular endothelial growth factor mRNA stability in hypoxia by heterogeneous nuclear ribonucleoprotein L
Shih S C and Claffey K P
The Journal of Biological Chemistry, 274(3), 1359-1365 (1999)
Matthew S Alexander et al.
Muscle & nerve, 63(6), 928-940 (2021-03-03)
RNA-binding proteins (RBPs) play an important role in skeletal muscle development and disease by regulating RNA splicing. In myotonic dystrophy type 1 (DM1), the RBP MBNL1 (muscleblind-like) is sequestered by toxic CUG repeats, leading to missplicing of MBNL1 targets. Mounting
Gracjan Michlewski et al.
RNA (New York, N.Y.), 16(8), 1673-1678 (2010-06-24)
RNA chromatography combined with mass spectrometry represents a widely used experimental approach to identify RNA-binding proteins that recognize specific RNA targets. An important drawback of most of these protocols is the high background due to direct or indirect nonspecific binding
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